Biotechnology and its Applications - NCERT based MCQs for NEET

This article contains NCERT based 45 MCQ questions test on Biology chapter "Biotechnology and its Applications". These questions are highly valuable for the NEET exam. You can attempt these questions in the form of an interactive quiz and calculate your score. Read the below quiz rules carefully before you start

General Instructions

• Quiz contains 45 questions of 4 marks each.
• Correct answer (✔) will award you +4 marks and Incorrect answer (✘) will give you -1 mark.
• Total test is of 180 marks.
• Tap on box in order to select any option that you think is correct.
• Press the Submit button given in the end of quiz to calculate your score.
• There is a PDF file attached in the end of quiz. You can see detailed solutions to all questions and do self analysis from that file as well. 

Question 1. A transgenic food crop which may help in solving the problem of night blindness in developing countries is

(1) Golden rice 

(2) Flavr Savr tomatoes

(3) Starlink maize 

(4) Bt soyabean

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Question 2. The proteins encoded by the genes cryIAc and cryIIAb control

(1) cotton bollworms 

(2) corn borer

(3) budworms 

(4) butterflies

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Question 3. Select the wrong statement.

(1) Human insulin is being commercially produced from a transgenic species of Escherichia coli.

(2) The genetically modified Bacillus thuringiensis is used as biopesticide on the commercial scale.

(3) Human protein, alpha-I-antitrypsin is used to treat emphysema.

(4) Bt toxin genes cryIAc control the corn borer

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Question 4. Which one of the following statements is wrong in relation to transgenic Bt cotton plant?

(1) Crop yield loss due to attack by Bacillus thuringiensis bacterium is reduced.

(2) Crop yield loss due to attack by lepidopteran insect pests is reduced.

(3) The use of chemical insecticides in the cotton field is minimized.

(4) Better quality cotton is produced.

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Question 5. A dicotyledonous plant forms crown gall when

(1) Agrobacterium tumefaciens comes in contact with the plant.

(2) Agrobacterium rhizogenes comes in contact with the plant.

(3) a specific part of DNA from the Ti plasmid gets integrated with the plant chromosome.

(4) a specific part of DNA from the Ri plasmid gets integrated with the plant chromosome.

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Question 6. Which of the following genes do not occur naturally in living organisms?

(1) Bt genes

(2) RNAi genes

(3) Cry genes

(4) Endogeneous cytoplasmic defense genes

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Question 7. Protein encoded by gene cryIAb controls the infestation of which of the following insects

(1) cotton boll worm 

(2) Anopheles mosquito

(3) corn borer 

(4) Aedes mosquito

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Question 8. The strategy used to prevent the nematode infection in the roots of tobacco plant is called

(1) use of agro chemicals 

(2) Bt toxin gene

(3) gene mutation 

(4) RNA interference

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Question 9. Bacillus thuringiensis (Bt) strains have been used for designing novel

(1) bio- fertilisers

(2) bio-insecticidal plants

(3) bio-mineralisation process

(4) bio-metallurgical techniques.

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Question 10. RNA interference which is employed in making tobacco plant resistant to Meloidogyne incognita is essentially involved in

(1) preventing the process of replication of DNA

(2) preventing the process of translation of mRNA

(3) preventing the process of splicing of hnRNA

(4) preventing the process of transcription.

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Question 11. Insect pest resistant Bt cotton plant was developed using

(1) somaclonal variation 

(2) micropropagation

(3) somatic hybridisation 

(4) transgenic technology.

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Question 12. RNA interference involves

(1) synthesis of cDNA & RNA using reverse transcriptase.

(2) silencing of specific mRNA due to complementary RNA.

(3) interference of RNA in synthesis of DNA.

(4) synthesis of mRNA from DNA.

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Question 13. Genetically modified (GM) crops can be produced by

(1) recombinant DNA technology

(2) somatic hybridisation

(3) cross breeding

(4) micropropagation.

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Question 14. RNA interference is essential for the

(1) cell proliferation 

(2) cell defence

(3) cell differentiation 

(4) micropropagation

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Question 15. Read the two statements A and B and identify the correct option from those given below.

Statement A: Agrobacterium tumefaciens is the causative agent of crown gall disease of dicots.

Statement B: Agrobacterium tumefaciens causes infection by entering the plant through wounds and injuries.

(1) Statement A is correct and B is wrong.

(2) Statement B is correct and A is wrong.

(3) Both statements A and B are correct.

(4) Both statements A and B are wrong.

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Question 16. This is not a GMO.

(1) Bt brinjal 

(2) Golden rice

(3) Tracy 

(4) Dolly

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Question 17. Which of the following is known as ‘Flavr Savr’?2

(1) Specific variety of pesticide

(2) Breed of chicken

(3) Transgenic tomato

(4) Toxic insecticidal protein

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Question 18. Golden rice is rich in

(1) vitamin B 

(2) vitamin A

(3) both (1) and (2) 

(4) none of these.

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Question 19. Basic principle of developing transgenic plants and

animals is to introduce the gene of interest into the

nucleus of

(1) somatic cell 

(2) vegetative cell

(3) germ cell 

(4) body cell.

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Question 20. The Bt toxin is not toxic to human beings because

(1) the pro Bt toxin activation requires temperature above human body temperature.

(2) the Bt toxin recognises only insect-specific targets.

(3) the Bt toxin formation from pre Bt toxin requires pH lower than that present in human stomach.

(4) conversion of pro Bt toxin to Bt toxin takes place only in highly alkaline conditions.

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Question 21. What is the source of the Ti (Tumor inducing) plasmid which is modified and used as a cloning vector to deliver the desirable genes into plant cells?

(1) Agrobacterium tumefaciens

(2) Thermophilus aquaticus

(3) Pyrococcus furiosus

(4) Aedes aegypti

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Question 22. Bt brinjal is an example of transgenic crops. In this, Bt refers to

(1) Bacillus tuberculosis 

(2) biotechnology

(3) β carotene 

(4) Bacillus thuringiensis

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Question 23. Which one of the following bacteria is used for production of transgenic plants?

(1) Escherichia coli

(2) Bacillus thuringiensis

(3) Staphylococcus aureus

(4) Agrobacterium tumefaciens

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Question 24. Salt tolerant transgenic has been developed for

(1) brinjal 

(2) grape

(3) potato 

(4) tomato

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Question 25. Insect tolerant gene from Bacillus thuringiensis is introduced using Ti plasmid of

(1) Escherichia coli

(2) Haemophilus influenzae

(3) Agrobacterium tumefaciens

(4) Arabidopsis thaliana.

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Question 26. cryIIAb and cryIAb produce toxins that control:

(1) cotton bollworms and com borer respectively.

(2) com borer and cotton bollworms respectively.

(3) tobacco budworms and nematodes respectively.

(4) nematodes and tobacco budworms respectively.

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Question 27. Which of the following is a transgenic plant?

(1) Flavr Savr

(2) Ashbya gossypii

(3) Meloidogyne incognita

(4) Gluconobacter oxidans

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Question 28. Bacillus thuringiensis is used as

(1) fungicide 

(2) weedicide

(3) biopesticide 

(4) insecticide

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Question 29. First genetically modified plant commercially released in India is

(1) golden rice 

(2) slow ripening tomato

(3) Bt-brinjal 

(4) Bt-cotton

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Question 30. The introduction of T-DNA into plants involves

(1) exposing the plants to cold for a brief period

(2) allowing the plant roots to stand in water

(3) infection of the plant by Agrobacterium tumefaciens

(4) altering the pH of the soil, then heat-shocking the plants.

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Question 31. Isolation of Bt gene from bacteriurn Bacillus thuringiensis was taken up in the year

(1) 1977 

(2) 1980

(3) 1997 

(4) 1990

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Question 32. In transgenics, expression of trans gene in target tissue is determined by

(1) enhancer 

(2) transgene

(3) promotor 

(4) reporter

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Question 33. Golden rice is a transgenic crop of future with following improved trait

(1) insect resistance 

(2) high lysine content

(3) high protein content 

(4) high vitamin A content.

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Question 34. Which kind of therapy was given in 1990 to a four year- old girl with adenosine deaminase (ADA) deficiency?

(1) Gene therapy 

(2) Chemotherapy

(3) Immunotherapy 

(4) Radiation therapy

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Question 35. In plant biotechnology, root tumors are induced in plant using the bacterium

(1) Agrobacterium rhizogenes

(2) Agrobacterium basilis

(3) Rhizobium

(4) None of the above

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Question 36. An example of gene therapy is

(1) production of injectable hepatitis B vaccine.

(2) production of vaccines in food crops like potatoes which can be eaten.

(3) introduction of gene for adenosine deaminase in persons suffering from Severe Combined Immuno Deficiency (SCID)

(4) production of test tube babies by artificial insemination and implantation of fertilized eggs.

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Question 37. What is the advantage in clinical use of humulin (human insulin produced through rDNA technique) over use of conventional ox or pig insulin?

(1) It does not cause immunological problems.

(2) It is cheaper for the patient.

(3) It is produced by E.coli in our own intestine.3

(4) There is no advantage.

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Question 38. The biological product created by the introduction of portions of DNA which codes for α-1 antitrypsin, is used to treat

(1) emphysema 

(2) asthma

(3) bronchitis 

(4) cystic fibrosis.

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Question 39. Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

(1) Genetic Engineering Approval Committee

(2) Research Committee on Genetic Manipulation

(3) Bio-safety committee

(4) Indian Council of Agricultural Research

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Question 40. How many recombinant therapeutics have been used for human diseases throughout the world?

(1) 12 

(2) 24

(3) 30 

(4) 56

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Question 41. The crops engineered for glyphosate are resistant/tolerant to

(1) insects 

(2) herbicides

(3) fungi 

(4) bacteria.

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Question 42. Ernst Chain and Howard Florey’s contribution was

(1) discovery of streptokinase

(2) establishing the potential of penicillin as an effective antibiotic

(3) discovery of the DNA sequencer

(4) isolating the bacterial plasmid

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Question 43. Human proteins can be produced in the milk or semen of farm animals. True or false?

(1) True.

(2) False, proteins cannot be produced in milk.

(3) False, proteins cannot be produced in semen.

(4) False, animals are not used for protein production.

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Question 44. The genetic defect, adenosine deaminase (ADA) deficiency may be cured permanently by

(1) administering adenosine deaminase through injection

(2) bone marrow transplantation

(3) enzyme replacement therapy

(4) introducing isolated gene from marrow cells producing ADA into the cells at early embryonic stages.

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Question 45. Fearing that the child to be born may have a genetic disorder, a couple goes to a doctor. Which one of the following techniques is likely to be suggested by the doctor to cure the genetic disorder?

(1) Hybridoma technology 

(2) Gene therapy

(3) rDNA technology 

(4) Embryo transfer

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Detailed Solutions

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